TransScript? Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)
耐高溫全預(yù)混第一鏈cDNA合成試劑盒（一步法去除gDNA）（用于qPCR）
| 目錄號 | 規(guī)格 | 單價 |
|---|---|---|
| AU341-02 | 100 rxns | 6110 |
產(chǎn)品詳情介紹
TransScript? Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)包含反轉(zhuǎn)錄反應(yīng)所需的全部成分(TransScript? Uni RT, RNase Inhibitor, Anchored Oligo(dT)20 Primer, Random Primer(N9), dNTPs, Buffer)，可在同一管反應(yīng)中實現(xiàn)cDNA 合成和去除基因組DNA。反應(yīng)時，只需加入5×All-in-One Reaction Mix for qPCR、TransScript? Uni All-in-One Enzyme Mix、模板RNA和水，在42℃-65℃條件下即可高效地合成第一鏈cDNA，同時去除RNA模板中殘留的基因組DNA 。另配有20×No RT Control Mix，用于配制無反轉(zhuǎn)錄酶的對照，判斷qPCR模板是否來自cDNA。該產(chǎn)品操作簡便，降低操作過程中的污染機(jī)率。cDNA產(chǎn)物只適用于qPCR，不適用于常規(guī)PCR。
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